FLAVONOID CONTENT AND ANTIBACTERIAL ACTIVITY OF ALBIZIA JULIBRISSIN. DURAZZ LEAF, STEM AND FLOWER EXTRACTS AGAINST CLINICALLY ISOLATED BACTERIAL PATHOGENS

摘要

Objective: To test the antibacterial efficacy of leaf, stem, and flower extracts of Albizia julibrissin against bacterial pathogens. Methods : Extraction of active metabolites was carried out by using six different solvents, and total flavonoid content in each extract was determined by Aluminium chloride method. To determine the antibacterial activity of extracts, disc diffusion method and tube dilution method were carried out. Zone of inhibition and Minimum inhibitory Concentration (MIC) were calculated. Results: Methanolic extracts of leaf samples of A. julibrissin showed highest extractive value (5.14g/100g) and total flavonoid content (35.14mg/g). In overall leaf extracts of A. julibrissin showed maximum zone of inhibition towards P. vulgaris (10.1 mm*) and least susceptible microorganism is S. typhi (3.5 mm*). Stem and flower extracts inhibited bacterial growth only at higher concentrations (MIC, 160-215 and 65-180μg/ml respectively). Conclusion : Apart from the energy crop, based on the results and value-added compounds present in A. julibrissin , it may be considered as antibacterial agent in future. Keywords : Albizia julibrissin, Antibacterial, Flavonoids.