ISOLATION, STRUCTURAL ELUCIDATION OF FLAVONOIDS FROM POLYALTHIA LONGIFOLIA (SONN.) THAWAITES AND EVALUATION OF ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER POTENTIAL

摘要

Thin Layer Chromatography (TLC) analysis of Ethanolic Extract of Polyalthia longifolia leaves (EEPL) revealed the presence of flavonoids and the results were also confirmed with Reverse Phase High performance Liquid Chromatography (RP-HPLC) analysis. The Fourier Transform Infra Red (FT-IR) spectra were recorded for Preparative Thinlayer Chromatography (PTLC) isolated samples in the range o f 4000 – 450 cm-1. The pronounced peaks belonging to the vibration of 3366 cm-1, 1650 cm-1and 679 cm-1 in the spectra corresponds to the characterized peaks of flavono ids. The High Performance Liquid Chromatography – Diode Array Detection – Electro Spray Ionization/ Mass Spectrometry (HPLC-DAD- MS (ESI+) analysis identified three flavonoids viz., Rutin, Chrysin and Daidzein-related isomer and an unknown flavonoid in the PTLC isolate of EEPL. In vitro anti-bacterial, anti-radical and MTT-based cytotoxicity activity against A549 human (lung carcinoma) cancer cell line study also strongly suggests that EEPL possess potent biological activity