Isolation, purification and structural elucidation of secondary metabolites from Microcystis aeruginosa bloom from Muttukadu estuary and its in vitro antibacterial, antioxidant and anticancer potency

摘要

The biomedical applications of the secondary metabolites produced by the harmful algal blooms were studied over a few decades ago. Only few research studies have been reported on the medicinal properties of the secondary metabolites produced by the cyanobacteria Microcystis aeruginosa. The present study was aimed to screen the Biological activities of GC fractionated Benzeneacetanomide and Norvaline, n-propargyloxycarbonyl mixtures of Microcystis aeruginosa secondary metabolites. The Microcystis aeruginosa samples were collected by plankton net from the blooming area at Muttukadu. The metabolites were extracted by using polar solvent as methanol. The yield percentage of Microcystis aeruginosa methanolic extract showed moderate level of 41% from the dry weight of the pre extracted Microcystis aeruginosa powder. The crude metabolites were purified through column chromatography and GC-MS analysis. The GC-MS results showed the presence of two compounds (2 major peaks), Benzeneacetamide at the retention time (RT) of 7.315 min and l-Norvaline, n-propargyloxycarbonyl (Pyrrol derivaties) at the retention time (RT) of 12.541 min. The GC-MS fractioned compounds were characterized by FT-IR and (HNMR)-H-1. The anticancer, antioxidant and antimicrobial activity of the compounds were studied. This study highlights the bioactive potential of Microcystis aeruginosa secondary metabolites that might lead to the development of cancer drug. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.