Screening of agricultural wastes for Glutaminase biosynthesis via Solid-state fermentation

摘要

An incredible increase in the world's population has led to the generation of million tons of agro-industrial wastes, which are in turn a great source of several bioactive metabolites such as therapeutic enzymes namely glutaminases. The enzyme has got immense potential applications across various industries varying from pharmaceutical to food. With the onset of scientific innovations, the usage of these nutritionally rich agricultural wastes for the synthesis of many valuable compounds significantly minimizing the production cost and pollution load on environment. The current investigation is aimed to explore and evaluate these inexpensive agro-wastes for glutaminase production under solid-state fermentation using Aspergillus wentii NCIM 661 and Fusarium oxysporum NCIM 1008. All the agro-wastes supported good microbial growth with better enzyme productivity. But among them, the maximum enzyme yield was noticed with wheat bran (9.36U/gds) using Aspergillus wentii NCIM 661, and sesame oil cake (10.27U/gds) with Fusarium oxysporum NCIM 1008, leaving the other substrates as noteworthy alternatives for the synthesis of glutaminase enzyme. This work had established the economical use of agro-industrial wastes into valuable metabolites which has considerable promising economics and environmental meaning.