IN VITRO CYTOTOXICITY AND GENOTOXICITY OF CHICKEN EGG YOLK ANTIBODIES (IGY) AGAINST TRYPANOSOMA EVANSI IN HUMAN LYMPHOCYTES

摘要

Objective: The present study aims to evaluate the cytotoxic and genotoxic effects of polyclonal antibodies (IgY), produced from the immunization of chickens against protozoan Trypanosoma evansi (T. evansi). Methods: For the tests a culture of human lymphocytes and IgY samples (1, 2.5, 5 and 10 mg/mL) were used. Cell viability was assessed by employing MTT assay, and the formation of reactive oxygen species (ROS) and lipid peroxidation were measured by testing the 2-thiobarbituric acid (TBARS). Genotoxicity was evaluated by chromosomal instability test. Results: At the concentrations tested, the study revealed that IgY anti-T.evansi showed no significant cytotoxic and genotoxic effects. Conclusion: These findings demonstrate the safety of these antibodies to mammalian cells