Culturing and PCR Methods for Detection of Mycoplasma hominis and Ureaplasma urealyticum in Women with Genitourinary Tract Infections

摘要

A total of Seven hundred and thirty five samples included; urine, endocervical, high vaginal and urethral swabs were collected from married 530/735 (72.1%) and unmarried women 205/735 (27.9%) who admitted to Al-Diwaniya Maternity and Pediatric Teaching Hospital, Al-Diwaniya Teaching Hospital and private clinics in Al-Qadisyia province, Iraq through a period of twelve months (from April 2011 to March 2012) in an attempt to detect the role of Mycoplasma and Ureaplasma in genitourinary tract infections in women. Culturing and PCR techniques were used in this study. Culturing method basing on diagnostic characteristics revealed positive results of Ureaplasma urealyticum in 352 cases while 91 cases were positive for Mycoplasma hominis in same method. According to the cases, the sources of isolates, 90 isolates of U. Urealyticum and 60 isolates of M. hominis were selected for PCR investigation. The results of this method indicated that 65/90 (72.2%) isolates of U. urealyticum were positive with PCR method versus 25/90(27.8%) were negative while among the 60 isolates of M. hominis, only 37/60 (61.7%) were positive with PCR technique versus 23/60 (38.3%) isolates exhibited negative results, accordingly, one can conclude that not all isolates being positive for culture can be positive for PCR too. These results evidently indicate that culturing methods for diagnosis of bacteria are still the confidential gold methods.