DETERMINATION OF ETILEFRINE HYDROCHLORIDE, FENOTEROL HYDROBROMIDE, SALBUTAMOL SULPHATE AND ESTRADIOL VALERATE USING SURFACE PLASMON RESONANCE BAND OF SILVER NANOPARTICLES

摘要

Objective : The aim of this work was to evaluate a simple, sensitive, effective and validated procedure for the determination of etilefrine hydrochloride, fenoterol hydrobromide, salbutamol sulphate and estradiol valerate. Methods : In this study the method based on the ability of the cited drugs to reduce Ag + ions to silver nanoparticles (Ag-NPs) in the presence of polyvinyl pyrrolidone (PVP) as a stabilizing agent producing very intense surface plasmon resonance peak of Ag-NPs (λ max = 417-425 nm). The plasmon absorbance of the Ag-NPs allows the quantitative spectrophotometric detection of the cited drugs. Results : The calibration curves were linear with concentrations range of 0.4-0.8, 0.1-0.9, 0.8-2 and 1.6-9.6 μg/ml for the cited drugs. Apparent molar absorptivity, detection and quantitative limits were calculated. Applications of the proposed methods to representative pharmaceutical formulations are successfully presented; also the proposed method was applied for the determination of estradiol valerate in human urine samples. Conclusion : The extracellular synthesis of nanoparticles was fast and eco-friendly; moreover, the method doesn't require various elaborate treatments and tedious extraction procedures. Keywords: Silver nanoparticles, Etilefrine hydrochloride, Fenoterol hydrobromide, Salbutamol sulphate, Estradiol valerate.