EVALUATION OF ANTIBACTERIAL POTENTIAL OF SILVER NANOPARTICLES (SNPs) PRODUCED USING RHIZOME EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG

摘要

Objective: The bio-reduction of silver ions in solution into silver nanoparticles by rhizome extract of Hedychium coronarium as capping, reducing and stabilizing agent. Further, to evaluate the antibacterial activity of the phytosynthesized silver nanoparticles. Methods: Aqueous extract of dried rhizome powder of Hedychium coronarium were used for the green synthesis of silver nanoparticles. The synthesized nanoparticle was characterized by UV-vis spectroscopy, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR) studies. Antibacterial activity of silver nanoparticles was carried out by disc diffusion method. Results: The observation of the peak at 428 nm in the UV-vis spectrum for phytosynthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. The SEM image shows that most of the phytosynthesized silver nanoparticles have spherical morphology. The average diameter of the particles was calculated from the XRD pattern and it was found to be 24 nm. Further, it was observed that silver nanoparticles have high antibacterial activity especially against gram negative organisms. Conclusion: The rhizome extract of Hedychium coronarium could be a good candidate for the green synthesis of silver nanoparticles. The antibacterial property of silver nanoparticles is a beneficial application in the field of medical nanotechnology. Keywords: Hedychium coronarium , Silver nanoparticles, Rhizome, SEM, FTIR.