ISOLATION OF PHYTOCONSTITUENTS AND IN VITRO ANTILITHIATIC BY TITRIMETIC METHOD, ANTIOXIDANT ACTIVITY BY 1,1- DIPHENYL -2- PICRYL HYDRAZYL SCAVENGING ASSAY METHOD OF ALCOHOLIC ROOTS & RHIZOMES EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG PLANT SPECIES

摘要

Objective: The herbal medicines do not have any side effect and it is cost effective and safest medicine from an ancient periods. Urolithiasis or Lithiasisis a consequence of complex physical processes. The major factors are supersaturation of urine with the offending salt and crystallization. Crystalsretained in kidney can become nucleus for stone formation. This process is synonymously known as Urolithiasis or Lithiasis or Nephrolithiasis orKidney stones or renal calculi. Calcium-containing stones are the most common comprising about 75% of all urinary calculi, which may be in theform of pure calcium oxalate (50%) or calcium phosphate (5%) and a mixture of both (45%). In this study, ethanolic & aqueous extracts of roots andrhizomes of Hedychium coronarium J. Koenig plant were evaluated for their potential to dissolve experimentally prepared kidney stones like calciumoxalate by titrimetic method with an invitro model and Antioxidant activity performed by DPPH scavenging assay method. Phytoconstituents werealso isolated by chromatographic techniques of this plant species.Method: For performing invitro antilithiatic activity titrimetic method was adopted and for antioxidant activity 1,1-diphenyl-2-picryl hydrazyl(DPPH) scavenging assay method was adopted. Phytoconstituents were isolated by column and thin layer chromatographic techniques.Results & Conclusion: Ethanolic roots & rhizomes extract of this plant produced highest dissolution of stones when compare to standard drugcystone and at 10 mg. concentration. Also this study showed alcoholic extract of roots & rhizomes of Hedychium coronarium J. Koenig plant in higherconcentration possess best antioxidant potential as compare to standard ascorbic acid with IC50 value 9.0 and 18.9 μg/ml. for ascorbic acid andalcoholic extract respectively. Isolated phytoconstituent from alcoholic extract of this plant was 8a, hydroxy hedychilactone and its structure wasconfirmed by IR, NMR and Mass spectroscopic datas.