STANDARDIZATION OF A POLYHERBAL PREPARATION (POL–6) FOR TREATMENT OF OXIDATIVE, INFLAMMATORY AND IMMUNE DISORDERS

摘要

Objective: The objective of this study was to develop and standardize a polyherbal preparation (POL–6) and evaluate its use in treating oxidative, inflammatory and immune disorders. Methods: The individual hydroalcoholic extractions of Hypericum perforatum, Bacopa monnieri, Centella asiatica, Withania somnifera, Ocimum sanctum and Camellia sinesis dried plant materials were done, and POL–6 was prepared by mixing them in (1:2:2:2:2:1) proportions. POL–6 has been standardized on the basis of organoleptic properties, physical characteristics, and physicochemical properties. The antioxidant activities were evaluated using free radical scavenging methods for 1,1–diphenyl–2–picrylhydrazyl, (DPPH) and nitric oxide assays. Anti–inflammatory activity was done by albumin denaturation and HRBC membrane stabilization assays. Cell viability assay was used for immunomodulatory activity measurement by cultivating peripheral blood mononuclear cells. Results: The results obtained from physical and chemical parameters evaluation shows that the contents of preparation present are within permissible limits . In in–vitro antioxidant studies POL–6 showed significant antioxidant activity when tested in 1–1 Diphenyl–2–picrylhydrazyl and nitric oxide assays. In in – vitro anti‐inflammatory investigation, POL–6 exhibited significant inhibition against albumin denaturation and significant protection against membrane stabilization assay. POL–6 also exhibit significant immunomodulatory activity at low doses, when tested in cell viability assay. Conclusion: The results from this study rationalize the medicinal use of POL–6 in oxidative, inflammatory and immune disorders. Keywords: Polyherbal preparation, Antioxidant, Anti‐inflammatory, Immunomodulatory