ANTIBIOTIC SUSCEPTIBILITY PATTERN AND ESBL PREVALENCE IN ESCHERICHIA COLI ISOLATES FROM PUS SAMPLES IN A TERTIARY CARE HOSPITAL

摘要

Objective: Escherichia coli is one of the most common etiological agent isolated from various infections. ESBL (Extended spectrum beta lactamase) producing strains of Escherichia coli have become a great therapeutic challenge to the clinicians in managing such infections. The aim and objective of the present study is to find out the antibiotic susceptibility pattern and to assess the prevalence of ESBL producers among the Escherichia coli isolates. Methods: 100 isolates of Escherichia coli from pus samples identified by standard conventional methods were included in the study. Antibiotic sensitivity testing was carried out using the Kirby Bauer’s disc diffusion method. Isolates showing resistance to third generation cephalosporins were subjected to phenotypic confirmatory test to confirm ESBL production. Results: The antibiogram revealed higher susceptibility percentage to Amikacin, Gentamicin and Levofloxacin. Higher resistance rate was noted for Amoxyclav, Ciprofloxacin, Cefotaxime, Ceftazidime and Ertapenem. All the isolates were found to be susceptible to Imipenem and Tigecycline. 36% of the Escherichia coli isolate were detected to be ESBL producers. Conclusion: ESBL producing strains of Escherichia coli will cause therapeutic failure and also contribute to multidrug resistance. Hence routine surveillance for ESBL production and infection control methods should be made mandatory. Keywords: ESBL (Extended spectrum beta lactamase), Escherichia coli , Ceftazidime and Ceftaclav (Ceftazidime clavulanic acid).