Antioxidant and Protective Effects of Green Tea against H2O2 induced Liver Injury in Rats

摘要

The study aims to identify green tea flavonoids and evaluate the protective effect of green tea water extracts against liver changes induced by H2O2 in male albino rats. Both two extracts showed a good amount of phenols and flavonoids; HPLC analysis of the different green tea flavonoids showed that epicatechin gallate, gallocatechin, caffeine, epicatechin and catechin are the main components in all green tea extracts. Rats were treated with H2O2 (0.5% in drinking water) and water extracts of green tea by stomach tube (80 mg/kg b.wt) for 30 days. Elevated levels of plasma total bilirubin, MDA and AST, ALT, ALP, GGT, SOD, CAT enzymes activity with decrease in plasma total protein and albumin levels in H2O2 treated rats compared with normal control group, all these parameters were significantly decreased by using different green tea extracts except total protein and albumin levels were significantly increased. Histopathological examination revealed degeneration of hepatocytes of rat liver treated with H2O2. Green tea extracts supplement improve the harmful effects of H2O2 in rats liver. Our results suggests that treatment with green tea extracts containing large amounts of phenolic compounds acting as natural antioxidants to reduce the harmful effect of treatment with H2O2.