Analysis of Bioactive Constituents from Organic Crude Ethanol Extracts from the Local Medicinal Plant of Cassytha filiformis L (Lauraceae) by Gas Chromatography - Mass Spectrometry”

摘要

Physiological changes in pregnancy, including changes in body composition and metabolic enzyme activity, can alter drug pharmacokinetics. Nifedipine (Adalat), a dihydropyridine calcium entry blocker, is an effective tocolytic agent with low toxicity and teratogenicity but has potential cardiovascular side effects that may affect the mother as well as the fetus. Therefore, the aim of this study was to assess the genotoxicity effects of maternal Nifedipine administration on micronucleus formation and DNA damage in bone marrow and their embryos. Swiss virgin female mice were allocated in several groups: control, non-pregnant female groups treated with three different doses of Nifedipine (25, 50 and 75mg/kg) and pregnant female mice treated with the same doses of Nifedipine starting from the day 3 to 18 of gestation. The results revealed that micronucleus formation and DNA damaged cells of non-pregnant and pregnant female mice as well as their embryos treated with low dose of Nifedipine (25 mg/kg) relatively similar to those in control cells. In contrast, the results showed that micronucleus formation and DNA damaged cells of non-pregnant and pregnant female mice as well as their embryos treated with medium and high doses of Nifedipine was higher 2 and 3 times compared with control and those treated with low dose of Nifedipine. The results suggest that induction of genetic toxicity by Nifedipine treatment was dose dependent manner. Nifedipine seems to be safe at the low dose but by increasing its concentration caused genetic toxicity due to imbalance in intracellular calcium level which increases of the ROS production.