Monoclonal Antibody EIV-E12 Recognizes a Glycoprotein Antigen, Which Differs from the B-Cell-Specific chB6 (Bu-1) Antigen

摘要

The antigen (s) recognized by the EIV-E12 monoclonal antibody (mAb) is first detected in the embryonic bursal mesenchyme on the precursor to the bursal secretory dendritic cell. Later in bursal embryonic development the prebursal stem cells which enter the developing follicle express the antigen recognized by mAb EIV-E12. The goal of this project is to characterize the antigen recognized by mAb EIV-E12. In western blotting the deglycosylated bursal proteins showed reduced reactivity to mAb EIV-E12, indicating, that the EIV-E12 epitope of the antigen may consist of a carbohydrate group that is common to chicken B-cells. The EIV-E12 positive antigen is expressed by the plasma cells of terminal maturation of B-cells unlike the B-cell specific chB6 antigen. These differences in the expression patterns of EIV-E12 and chB6 positive antigens are confirmed by their different molecular weights of 200 and 65 kDa, respectively.