First Preliminary Report on Isolation and Characterization of NovelAcinetobacterspp. in Casing Soil Used for Cultivation of Button Mushroom,Agaricus bisporus(Lange) Imbach

摘要

Despite evaluation of large number of agroindustrial wastes for their use as casing material forAgaricus bisporus(Lange) Imbach cultivation, scant attention has been given to the importance of biological properties of casing materials. In the present study, an attempt was made to characterize the bacterial flora in casing layer, namely, Farm Yard Manure (FYM) and Spent Mushroom Substrate/spent compost (SMS/SC) (FYM+SC, 3 : 1) and FYM and Vermi Compost (VC) (FYM+VC, 3 : 1), employing partial 16S rDNA sequencing. Available data showed a significant variety of organisms that includedAcinetobacterandPseudomonasof theγ-proteobacteria, that were the most frequently encountered genera. This is the first preliminary report on the microbial diversity of casing soils and demonstrates the presence ofAcinetobacterspp. that has not been previously described in casing material.