Induction of Somaclonal Variation in Selected Drought Sensitive Genotype of Sugarcane (Sachharum officinarum)

摘要

In this research study, induction of somaclonal variation in selected sugarcane genotype, CPF-248 was investigated. For this purpose, five media for callogenesis were studied and embryogenic, fragile calli were achieved on CIM3 (7.75 mg/L 2, 4-D). So, calli of three different age groups; 35 days old calli,45 days old calli and 55 days old calli, induced on this callus induction medium (CIM3) were shifted to four regeneration media. Among these media few plants were achieved from 35 days old calli on shifting to RM1 (BAP 3.25 mg/L and casein hydrolysate1.75 mg/L). Hence, for in vitro selection of drought tolerant somaclonal variant plants, RM1 was supplemented with 7% PEG (IVSM1), 8% PEG (IVSM2), 9% PEG (IVSM3) and 10% PEG (IVSM4). On these media, two plants were achieved and named as in vitro selected putative drought tolerant somaclonal variants (IPSV). These plants were then subjected to genomic as well as physiological analyses. Genomic analysis revealed that the plant obtained from IVSM1 (IPSV1) showed different DNA banding pattern to its parent plant while plant from IVSM2 (IPSV2) gave same DNA profile as parent genotype (CPF-248). Whereas physiological analyses showed IPSV1 has more water potential, osmotic potential, turgor potential, CO2 assimilation rate, transpiration rates water use efficiency and relative leaf water contents as compared to its parent genotype as well as IPSV2.