The electrochemical impedance spectroscopy, EIS, was used to investigate the interfacial behavior ofbovine serum albumin (BSA) on different titanium (Ti) surfaces at open circuit potential. Nano poroustitanium oxide film, TiO2, was anodically formed on titanium from electrolyte containing 1M H2SO4and 0.5% wt NaF at 20 V for 20 min. The biomimetic calcium-phosphate coating was depositedcathodically on anodized titanium, (AT) from supersaturated calcium-phosphate solution, SCPS, atlow temperature, 60C. To be the Ti, AT, and Ca-P/AT surfaces have been used to compare amongmicrostructures and protein adsorption behavior. Island from calciumhosphate coating was detectedby the scanning electron microscope, SEM. The electron diffraction X-ray analysis, EDX, was used toinvestigate the difference in chemical structures between different types of examined surfaces. Theexperimental impedance data were fitted to theoretical data according to proposed equivalent circuitmodels. The impedance data fitting enabled to explain the adsorption phenomena of BSA occurred onthe different specimen surface. This work indicated formation of nano-TiO2 film on Ti improved theCa-P induction ability which enhanced the adsorption of BSA on its surface.
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