A novel electrochemiluminescnece sensor based on an Ru(bpy)₃2+ - Eu₂O₃ - nafion nanocomposite and its application in the detection of diphenhydramine

摘要

An ultrasensitive solid-state electrochemiluminescence (ECL) sensor based on the efficient and stable 2+ enhancement of tris-(bipyridine)ruthenium(II) (Ru(bpy)3 ) by diphenhydramine (DPH) in a carbon paste electrode has been developed. The ECL intensity was found to be correlated to the concentration of DPH. Incorporation of europium oxide (Eu2O3) nanoparticles in the carbon paste electrode (CPE) led to the development of the novel ECL sensor. Eu2O3 showed attractive sensitizing effects for the 2+ Ru(bpy)3 -DPH electrochemiluminescence system. Under optimized conditions, the linear response of the ECL intensity to DPH concentration was in the range of 1.0×10-9 to 1.25×10-7mol/L (R2= 0.998) with a detection limit (S/N=3) of 3.0×10-10mol/L and a relative standard deviation (RSD) of 4.1%. The evaluations proved the method as being very sensitive, selective and simple. The sensor was also used in the analysis of DPH concentration in serum and urine specimens.