Development of a Cyclic Voltammetry Method for DNA Electrochemical Detection on Microfluidic Gene Chip

摘要

On the microfluidic gene chip, due to high difficulty in temperature changes frequently and productsdetecting equipment miniaturize, the conventional methods of DNA detection can meet therequirements. In this paper, a newly electrochemical method, cyclic voltammetry, basing on a set ofspecial electrodes and the Loop-mediated isothermal amplification (LAMP), was introduced. TheDNA amplification products of LAMP could be combined with the positive dye (Methylene blue),which leading to a reduction in the oxidation peak current (ipA) and reduction peak current (ipC) ofthe cyclic voltammetry. The changes of ipA/ipC were real-time measured by the special electrodes, sothe copies of DNA were quantitative detected. The results show that it could be completed in 300-1 minutes with the lowest DNA to 10 CFU.mL , with high accuracy (96.5%), high sensitivity (96.0%),high specificity (97.0%) compare to the PCR, and good anti-interference ability against Vitamin C (up-1 -1 to 32mg.L ) and Aspirin (up to 64mg.L ) within the sample. Therefore, it was a rapid, sensitive andstable method of DNA detection, has great potential in applying on the microfluidic gene chips.