MICROFLUIDIC CHIP FOR HIGHLY SENSITIVE SIGNAL DETECTION OF DUPLEX DNA MIXTURES USING CONFOCAL SURFACE ENHANCED RAMAN MICROSPCOPY AND THE DETECTION METHOD THEREOF

摘要

The present invention relates to a micro-fluidic chip and a DNA mixture optical detection method using the same which is used to optically detect the DNA adsorbed onto the DNA mixture on the nanoparticles, and more particularly, is to mix efficiently the nano-colloid and the DNA mixture micro-fluidic chip, which relates to Dick and method for quantitative detection of the DNA mixture by an optical detection method of the confocal SERS using the same. ; The PDMS polymer material is patterned on a silicon substrate using a semiconductor process, and then to produce a lab-on-a-chip having the shape of a crocodile tooth channel (channel). The micro channel of the fabricated raepchip is injected into the nanoparticle colloid with two DNA mixture measures the amplified signal by scanning the argon ion laser was adsorption of DNA molecules on the nanoparticles. At this time, the measurement limit of the measured DNA mixture is capable of long, 10 ^-12 M, a low-concentration observed. The highly sensitive light scattering amplification technique can be widely used in high-sensitivity measurement of the biological sample, such as DNA and proteins in the raepchip. ; Lab-on-a-chip, microfluidic chips, Raman spectroscopy, SERS, PDMS, confocal