Molecular Cloning and Analysis of Glycoprotein Genes C, D and I of an Isolated Strain of Marek's Disease Virus Type-3

摘要

Marek's disease is the most commonly occurring neoplasm of any animal population and until recently it has caused extensive economic loss globally. Marek's virus, an actively evolving virus is the causative agent. Now with the virus's genetic code in hand we could easily characterise the immunogenic proteins of the closest relative of the virus, the Herpes virus of Turkey's.The important glycoprotein genes gC, gD,and gI of an isolated strain of Herpes virus of Turkey's were amplified, cloned in pGEM T-Easy vector and sequenced as an initial step in developing a recombinant vaccine against Marek's Disease. The recombinant genes were analysed and sequence data confirmed that the isolated strain was HVT (FC126), a vaccine strain.