Methods for producing a continuous cell line, for generating and / or isolating and / or maintaining a marek disease virus strain, for producing a diagnostic antigen for a marek disease virus, for preparing a vaccine capable of inducing protection against disease in a vertebrate, and to protect a vertebrate against disease, a cell or its offspring, cell preparation, use of it, vaccine, and use of it

摘要

"METHODS FOR PRODUCING A CONTINUOUS CELL LINE, TO GENERATE AND / OR ISOLATE AND / OR MAINTAIN A MAREK DISEASE VIRUS CUP, TO PRODUCE AN ANTIGEN DIAGNOSIS OF MAREK DEACPAR VARAZA PREPARE VIRUS DISEASE AGAINST DISEASE IN A VERTEBRATE, AND TO PROTECT A VERTEBRATE AGAINST DISEASE, CELL OR ITS PROLET, PREPARATION OF CELLS, USE OF THE SAME, VACCINE, AND, USE OF THE SAME ". The invention provides a method for producing a continuous cell line capable of supporting the growth of a Marek's disease virus strain comprising infecting or transfecting a cell with a vector comprising a nucleic acid or fragment thereof of a disease virus strain. of Marek and culturing said infected or transfected cell or its offspring under conditions suitable for expression of said nucleic acid and propagation of said cell or its offspring. Preferably, said nucleic acid or fragment thereof comprises a Marek's disease virus glycoprotein gE gene or functional fragment thereof. The invention further provides a method for generating and / or maintaining a hypervirulent and / or very virulent, and / or very virulent, and / or virulent and / or avirulent strain of Marek's disease virus comprising infecting a cell according to the invention. with a hypervirulent, and / or very virulent, and / or very virulent plus, and / or virulent and / or avirulent strain of Marek's disease virus and culturing said cell under conditions suitable for propagation of said cell and generation, maintenance and isolating said hypervirulent, and / or very virulent, and / or very virulent plus, and / or virulent and / or avirulent strain of the Marek's disease virus. The invention further provides a method of preparing a vaccine capable of inducing protection against disease, preferably a disease associated with Marek's disease virus in a bird comprising cultivating a continuous cell according to the invention and harvesting from it cell culture components. .