Antibacterial activity of bacteriocin isolated from Lactobacillus acidophilus against throat infections causing bacteria

摘要

The present study was designed to illustrate the antibacterial activity of bacteriocin against antibiotic resistant bacteria isolated from throat of patients with upper respiratory tract infections. Throat swabs of 439 patients were collected from local population of District Sargodha and employed in the study. Bacteriocin was conjugated antibiotic and isolated from L. acidophilus. It was purified using ammonium sulphate precipitation method. It was found stable at different temperatures and specific pH range. First, antibiotic susceptibility was checked against widely used antibiotics (Ciprofloxacin, Levofloxicin, Amoxicillin and Erythromycin). Then, bacteriocin sensitivity assay was performed using disc diffusion technique and agar well diffusion assay. Other than commonly reported strains; three strains (Moraxella catarrhalis, Neisseria meningitides and Alcaligenes sargodhrensis) were associated with the upper respiratory tract infection in the local population of District Sargodha. Alcaligenes sargodhrensis was novel strain and identified using 16S rRN Aribotyping. All strains were resistant to selected antibiotics at certain concentrations. Bacteriocin was effective against all test antibiotic resistant strains. This study suggested that the developing resistance in respiratory tract pathogens against cultural antibiotics can be minimized with the alternative strategies such as bacteriocins.