嗜酸乳杆菌细菌素的分离纯化及表面活性剂对其活性的影响1)

摘要

从本研究室保藏的一株来自于凯菲尔粒的嗜酸乳杆菌培养基质中分离出具有抑菌活性的蛋白组分———细菌素，对该嗜酸乳杆菌所产生的细菌素的分离方法及表面活性剂对其抑菌活性的影响作用进行了研究。结果表明，嗜酸乳杆菌在添加了油酸的 MRS 培养基中培养16h 后达到产细菌素高峰；对细菌素分离纯化研究包括三个步骤：硫酸铵沉淀、离子交换色谱(IEC)和疏水作用色谱(HIC)。不同类型的表面活性剂对细菌素的抑菌活性具有不同的影响。%　　Bacteriocin which is a kind of protein with antibacterial activity is produced from L actobacillus acidophilus ,and the strain is isolated from kefir grain preserved in the lab .The separation method about bacteriocin and the influence of the different surfactants on the antibacterial activity of bacteriocin is stud -ied .The results show that L actobacillus acidophilus is cultured in MRS medium addind oleic acid after 16h culture ,a peak of the bacteriocin-producing is reached .And The purification method about bacterio-cin includes three operations ,which separately are ammonium sulfate precipitation ,ion exchange chroma-tography(IEC)and Hydrophobic Interaction Chromatography . The different kinds of surfactants may have the different effects on the antibacterial activity of bacteriocin .