Does the MTHFR A1298C PolymorphismAssociate with Coronary Artery Disease inPatients from Haryana?

摘要

Background and Aim: Various risk factors are associated with the development of Coronary Artery Disease (CAD) whichincludes both modifiable and non-modifiable factors including genetic factors. MTHFR polymorphism may be an importantgenetic factor for the development of CAD. Therefore, in view of paucity of information from Haryana, the study has beendesigned to assess the risk of MTHFR A1298C polymorphism in CAD patients from Haryana.Materials and Methods: The 97 (56 males, 41 females) CAD patients and 93 (males 51, females 42) healthy controls of 21-50years of age were included in the study. The risk factors, lipid profile and clinical parameter were recorded in a predesignedperforma. DNA was extracted from blood and PCR-RFLP was done to detect the MTHFR A1298C polymorphism.Results: MTHFR A1298C polymorphism denoted as AA, AC and CC genotypes. No significant difference (p>0.05) has beenobserved in the distribution of MTHFR A1298C polymorphism in CAD patients in comparison to healthy controls. Thedistribution of MTHFR A1298C polymorphism has been found to be independent of age and sex. The difference in thedistribution of MTHFR A1298C polymorphism among young age (≤40 years) CAD patients also found to be statistically nonsignificant (p>0.05). These results rule out the probability of early onset of the disease due to MTHFR A1298C polymorphism.The difference in the incidence of MTHFR A1298C polymorphism (AA, AC and CC) in CAD patients with positive family historyfound to be statistically non-significant (p>0.05).Conclusion: The distribution of MTHFR A1298C polymorphism among CAD patients from Haryana is independent of age, sexand family history. The study revealed no association of MTHFR A1298C polymorphism with the development of CAD inpatients from Haryana; therefore, rule out the probability of MTHFR A1298C polymorphism as an independent risk factor forCAD.