Comparison of Mouse Embryo Cleavage Rate from One Cell Stage to Blastocyst Stage in Co-culture with Vero Cells and Without Co-culture

摘要

The aim of this study was to evaluate the effects of Vero cells on early embryonic cleavage rate and overcome cellblock of mice embryos in vitro . Female mice were super-ovulated by Intra-peritoneal injection of 5 IU Pregnant mare serum gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (HCG) 48 h later. The super-ovulated female NMRI mice placed individually with NMRI (Noda Medical Research Institute) males of proved fertility. The following morning, the females with positive vaginal plug were killed and cumulus-enclosed single cell embryos (2PN) were recovered. Two pronuclear (n=170) embryos were divided randomly into 2 groups: (1) co culture with vero cells (2) culture in simple culture medium. The rate of the development and the morphological appearance of mouse embryos in two groups were recorded daily for 120 h after retrieval in each system using inverted microscope. On day 5 of development the results showed that embryos cultured on vero cells had a significantly higher blastocyst and hatching formation rate than those in simple culture medium alone (p<0.05). It is concluded that Vero cells may improve mouse embryo development partly by increasing blastocyst formation, hatching blastocyst rate.