Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1

摘要

Objective: Our study aims to investigate the effect of arsenic trioxide (As 2 O 3 ) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells. Methods: Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As 2 O 3 were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3?μmol/L As 2 O 3 . The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively. Results: As 2 O 3 of different concentration could inhibit the proliferation of Tca8113 cells and IC 50 value was 3.004?±?0.2379?μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3?μmol/L As 2 O 3 for 48?h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As 2 O 3 on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells. Conclusion: As 2 O 3 could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.