The correlation of production characteristics with the genetic variants of the encoding locus of ?-lactoglobulin in three sheep breeds from Romania

摘要

The aim of this study is the genetic characterization of the β-lactoglobulin locus in the indigenous sheep breeds Karakul of Botosani, Merino Palace and Palace milk line. For animal breeders, the genetic polymorphism of milk proteins is of great interest because of milk composition and quality and yield parameters. The polymorphism of the β-lactoglobulin locus was analyzed in three breeds using the RFLP technique. In order to perform this analysis, we designated primers to amplify the polymorphic region at the level of the encoding gene for the β-lactoglobulin protein and the fragments amplified were subjected to endonuclease Rsa I digestion. The fragments resulting from restriction were analyzed by agarose gel electrophoresis, identifying thus the allele variants present. The results of the study suggest the possibility of a selection process induced in order to improve the production performance for milk. The using of genetic markers in animal selection is a successful strategy for production systems, the result of applying such methods leads to increasing productivity, product quality and of the benefits obtained in the field of livestock farming