Influences of Explant Type on in vitro Regeneration of Malaysian Chilli (Capsicum annuum L.) var CB 4

摘要

Regeneration system via organogenesis has been developed for Malaysian chilli ( Capsicum annuum L) var. CB4. Hypocotyls, cotyledons and petioles from In vitro germinated seedlings were cultured on medium comprising of Murashige and Skoog’s basal medium supplemented with 5 mg/l BAP, 1 mg/l IAA and 25 g/L DJ nutrient. Hypocotyls and petioles were found to be the best explants, based on its ability to produce shoot buds compared to cotyledons. Shoot buds were elongated on MS medium containing 3 mg/l BAP, 1 mg/l IAA, 15 g/L DJ, 2 mg/L GA3, 10 mg/l AgNO3 and 15 g/L DJ nutrient. Rooting was induced on MS basal containing IAA.