Pyrosequencing investigation into the influence of Cu2+, Zn2+, Fe2+ and I? mixtures on fungal diversity and toxigenic fungal growth in a fermented liquid feed

摘要

Abstract A L9(34) orthogonal experiment was conducted to evaluate the influence of 9 mixtures which consisted of Cu2+, Zn2+, Fe2+ and I? ions at different ion concentrations on fungal diversity and toxigenic fungal growth in a Bacillus subtilis-fermented liquid feed (FLF) using pyrosequencing. The maximal Chao estimator and Shannon index were achieved in the {FLF} with a mixture of Cu2+ (200?mg/kg), Zn2+ (160?mg/kg), Fe2+?(150?mg/kg) and I? (2.4?mg/kg). The minimal relative abundance of Aspergillus was achieved when a mixture of Cu2+ (200?mg/kg), Zn2+, Fe2+ and I? was added to the FLF. Compared with Zn2+, Fe2+ and I?, Cu2+ was the most important ion in inhibiting Aspergillus growth. Adding Zn2+ (160?mg/kg), Cu2+, Fe2+ and I? to the {FLF} minimized the relative abundance of Fusarium. It was Zn2+ instead of Cu2+ played a critical role in suppressing the growth of Fusarium. The proper use of the mixture of Cu2+, Zn2+, Fe2+ and I? in {FLF} contributes to inhibit the growth of mycotoxin-producing fungi during storage. The new findings of this study help farmers properly use the mixture of Cu2+, Zn2+, Fe2+ and I? to inhibit the growth of mycotoxin-producing fungi in the production of high quality {FLF} and alleviate mycotoxins damages to animals and humans.