Effect of Hyaluronic Acid (HA) on Organogenesis in Protocorm-Like Bodies(PLBs) of Phalaenopsis ‘Fmk02010’ Cultured in vitro

摘要

Phalaenopsis orchids have high economic value in the floriculture industry as cut flowers and even more as potted plants. The aim of this study is to integrate findings on the regulatory function of Hyaluronic acid (HA) on growth and development of protocorm-like bodies(PLBs) in Phalaenopsis ‘Fmk02010’. Results of this study revealed that low concentration of HA in modified MS medium increased maximum formation of PLB within short duration of culture. In Phalaenopsis, 100% PLB formation rate was observed on all the medium containing HA9 or HA12 except high concentration after six weeks of culture. In, Phalaenopsis, 93.3% shoot formation rate was observed on media containing 0.1 mg/L HA12. Based on our present study, it could be concluded that low concentration of Hyaluronic acid added with culture medium, rapidly increased the formation rate of PLBs and shoots in vitro culture of Phalaenopsis ‘Fmk02010’.