Effect of 6-Ben/y laminopurine (BA) and Hyaluronic Acid (HA) under White Light Emitting Diode (LED) on Organogenesis in Protocorm-Like Bodies (PLBs) of Dendrobium kingianum

摘要

Growth and development of PLBs cultured in vitro largely dependent on the presence of different plant growth regulators. In this study, potential effect of BAand hyaluronic acid (HA9; Shiseido, Japan) on organogenesis of protocorm-like bodies (PLBs) in Dendrobium kingianum under white light emitting diodes(LEDs) was investigated. PLBs of D. kingianumwere explanted on modified MS medium supplemented with various concentration of BA and HA9. The results indicated that the application of BA at low concentration (0.1 g/L) increased average number of PLBs, shoots and their fresh weight. In case of HA9, the highest number of PLBs (17.5) was recorded at concentration of O.lmg/L. While the highest number of shoots per explant (3.6) and the percentage of shoot formation (66.7) were observed at concentration of lmg/L HA9. This study shows that low concentration of BA is a potential plant growth regulator and HA9 acts as a plant growth regulator like BA for organogenesis in PLBs of Dendrobium kingianum.