Effect of ultrasonicated water treated with quartz porphyry 'Bakuhan-seki' on organogenesis in protocorm-like body (PLB) of Cymbidium and Phalaenopsis species cultured in vitro

摘要

Effects of ultrasonicated water treated with quartz porphyry "Bakuhan-seki" (UW-B) added on organogenesis in protocorm-like body (PLB) of Cymbidium and Phalaenopsis species cultured in vitro were examined. The medium supplemented with 2 percent UW-Bbrought good results on PLB formation of these species. The best results for inducing shoot with roots in PLB cultures were obtained by medium with 1 percent UW-B (C. insigne) or 2 percent UW-B (Phalaenopsis). UW-B added to culture media was found to beefficient for proliferation of PLBs and subsequent shoot formation of both Cymbidium and Phalaenopsis cultures.