Screening of Antioxidant and Radical Scavenging Activity of Vigna ungiculata , Bidens pilosa and Cleome gynandra

摘要

This study was conducted to investigate the total phenolics content and the antioxidant activities from m ethanolic extract s of Vigna ungiculata , Bidens pilosa and Cleome gynandra . Antioxidant and free radical scavenging activity were studied using DPPH and reducing power assays, a?2-carotene linoleic acid model system and the inhibition of lipid peroxidation in rat brain. Phenolic compounds were also quantified using HPLC. Total phenolic compounds for Vigna ungiculata , Bidens pilosa and Cleome gynandra were 1136.603?±3.869 g g^-1, 1102.797?±2.239 mg g^-1 and 1327.333?±1.658 mg g^-1 dry mass, respectively. All three vegetable extracts contained vanillin, caffeic acid, p-coumaric acid and ferulic acid. There was an increase in the reducing power effects and inhibition of lipid peroxidation in all samples. All samples showed a time dependent decrease in radical scavenging of DPPH and?2-carotene.