Antimicrobial inhibition on zoonotic bacterial Escherichia coli O157: H7 as a cause of food borne disease

摘要

This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7. The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.