Evaluation of the lethal dose of the methanol extract of rhizophora racemosa leaf using Karbers method

摘要

Aim: To evaluate the median lethal dose (LD50) of the methanol extract of Rhizophora racemosa leaf commonly used for toothache and dysmenorrhoea in the Niger Delta Area of Nigeria.Methods: The modified arithmetic Karber’s method by Aliu and Nwude was used. Various doses of the extract were administered to adult albino rats through intraperitoneal route and the LD50 was calculated.Results: The result revealed that the LD50 of the methanol extract was 1583.33 mg/kg which is within safe level of consumption.Conclusion: This research therefore provides a scientific evidence that the traditional medicinal use of this plant is safe within the dose limit established.Keywords: LD50, Rhizophora racemosa, Mangrove plant, Karber’s method, Intraperitoneal.