Comparison of ompP5 sequence-based typing and pulsed-filed gel electrophoresis for genotyping of Haemophilus parasuis

摘要

In this study, comparison of the outer membrane protein P5 gene (ompP5) sequence-based typing?with?pulsed-field gel electrophoresis?(PFGE) for the genotyping of?Haemophilus parasuis, the?15?serovar?reference strains and 43 isolates?were investigated. When comparing the two methods,?31?ompP5?sequence types?(STs)?and 43 PFGE types?were identified?withompP5?STs?A16 and A28?being the?dominant?ompP5?ST types, while?no predominant PFGE type was found.?The discriminatory indices?were?0.95?for?ompP5?sequence-based method and 0.98 for PFGE, and?the two techniques were proved to be?70.7% congruent. Therefore,ompP5?sequence-based typing?was a simple?and inexpensive method, enabling it as a preliminary technique to research the molecular differentiation of?H. parasuis.