The Comparative Study of 8Hz EMF Effect on Tissue Hydration in Brain Cortex and Subcortex of Rats

摘要

Previously we have shown that 8Hz EMF has depressing effect on thermodynamic activity of water, which decreases peroxide formation, and 8Hz EMF-treated aqua solution depresses the growth and development of microbes in aqua medium. Therefore, in order to elucidate the possible mechanism of 8Hz EMF on brain function, in the present work the studies of 8Hz EMF exposure effect on brain tissue hydration, the sensitivity of 8Hz EMF-induced tissue hydration to 10~(-4)M ouabain~( )(Na~(+)/K~(+) pump inhibition) and 10~(-9)M ouabain (activation of intracellular signaling system) as well as 8Hz EMF exposure effect on the number of Na~(+)/K~(+) pump units in the membrane of both young and old rats have been performed. The obtained data allow us to suggest that 8Hz EMF exposure has age-dependent modulation effect on brain tissue hydration and this effect is sensitive to Na~(+)/K~(+) pump activity and intracellular signaling system. It has been shown that, in spite of the fact that the number of expressed Na~(+)/K~(+) pump units in cell membrane of subcortex is significantly less expressed than in cortex cells, the sensitivity of signaling system controlling cell hydration to 8Hz EMF in subcortex cells is more pronounced than in cortex ones.