Bioprocess conditions of Acetic Acid production by using Acetobacter cerevisiae in shake flask fermentation

摘要

The current research designed the improve acetic acid production by using Acetobacter cerevisiae through enhancement of the generation medium. Through this research, medium contain of [g/L]:Yeast extract 6, peptone 5, glucose 20, ethanol 25,acetic acid 15, MgSO 4 .7H 2 O 0.57, K 2 HPO 4 1.5, FeSO 4 .7H 2 O 0.03, and MnSO 4 .4H 2 O 0.12. The procedure improvement was begun by enhancement of medium synthesis utilizing one factor at time and affected on carbon and nitrogen sources, also affect the cell dry weight (CDW) on acetic acid yield and screening compering between developments through un-optimized and optimize medium in the shake flask stage. Result demonstrated that, the cell dry weight through optimized medium and un-optimized medium was9.10 [g/L] and 4.38 [g/L], individually and the acetic acid through optimized medium and un-optimized medium was 13.5[g/L], 5.0[g/L], respectively. All the results derived from this research have been found to be optimization medium was developed Acetic acid production.