Effect of pH, temperature and incubation time on cordycepin production from Cordyceps militaris using solid-state fermentation on various substrates

摘要

ABSTRACT Cordyceps militaris has been a keystone in combating myriad health problems with innumerable far-reaching therapeutic effects. The present study focuses on effect of fermentation conditions such as (pH, temperature and incubation time) and solid-state fermentation (SSF) using solid substrates (wheat, oat and rice) on production of cordycepin. Temperature, pH and incubation time was found to have a direct effect on cordycepin production. The best possible combination of temperature, pH and incubation time was found to be 25???°C, 5.5 and 21????days, respectively, for maximum cordycepin production. SSF of solid substrate medium culture leads to the production of cordycepin. Among the solid substrates, rice medium had highest cordycepin production (814.60????mg/g) followed by oat and wheat medium (638.85 and 565.20????mg/g, respectively). This method provides an effective way for increasing the cordycepin production at a large scale. This study could have a wide application in other fermentation processes at industrial level.