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DNA Profiling of Seed Parents and a Topcross Tester and its Application for Yield Improvement in Timothy ( L.)

机译:种子亲本和Topcross检测仪的DNA分析及其在提摩西(L.)产量提高中的应用

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Assessment of genetic diversity based on DNA profiling contributes to the selection of superior parents for heterosis. The objective of this study was to investigate how closely the yields of timothy (Phleum pratense L.) topcross progenies are related to the genetic diversity between their seed parents and the pollen tester parent a€?Auroraa€?, and to predict their yield potentials from the index based on simple sequence repeat (SSR) marker polymorphisms. Genetic diversity among 67 genotypes, which consisted of 16 plants of timothy cultivar Aurora, the pollen parent for topcross testers, and 51 clones derived from breeding populations was evaluated based on 28 SSR markers carrying 408 alleles. Genetic distance (GD) was estimated for all possible pairs of genotypes and then the mean of the GD estimates between each clone and Aurora (GDA) was also assessed to find the relationship between GDA estimates and yields of topcross progenies derived from all crosses between each clone and Aurora. The GDA estimates of the clones ranged from 0.653 to 0.756, and a correlation coefficient between the GDA estimates and yields of their topcross progenies was 0.675 (p 0.001). These results strongly suggest that GD would provide reliable information on the yields of the assumed topcross progeny lines and that it will help breeders to accelerate yield improvements for a practical breeding program of timothy.
机译:基于DNA分析的遗传多样性评估有助于选择优势亲本的杂种优势。这项研究的目的是调查提摩太(Phleum pratense L.)topcross后代的产量与其种子亲本和花粉测试亲本(Auroraa)之间的遗传多样性有多紧密的联系,并预测其潜在的产量。基于简单序列重复(SSR)标记多态性的索引。基于28个带有408个等位基因的SSR标记,评估了67种基因型之间的遗传多样性,这些基因型由16种提摩斯品种Aurora,花粉亲本作为顶交测试者的花粉亲本组成。估计所有可能的基因型对的遗传距离(GD),然后评估每个克隆和Aurora(GDA)之间的GD估计值的平均值,以发现GDA估计值与源自每个基因之间的所有杂交的Topcross后代产量之间的关系克隆和极光。克隆的GDA估计值介于0.653至0.756之间,并且GDA估计值与它们的上交子代产量之间的相关系数为0.675(p <0.001)。这些结果强烈表明,GD将提供有关假定的topcross后代品系的产量的可靠信息,并且它将帮助育种者加快提摩太鱼的实际育种程序的产量提高。

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