Analysis of the volatile constituents ofApium graveolens L. andOenanthe L. by gas chromatography-mass spectrometry, using headspace solid-phase microextraction

摘要

Gas chromatography-mass spectrometry with headspace solid-phase microextraction was used for the analysis of the volatile constituents of celery and water dropwort. Solid-phase microextraction was carried out with a 65 μm polydimethylsiloxane-divinylbenzene fiber at 60°C for 45 min. The fiber was desorbed in the GC injection liner at 250°C for 3 min. Thirty-eight compounds in celery and thirty-five compounds in water dropwort were separated and identified. The main compounds found in both celery and water dropwort includedcis-3-hexen-1-ol, α-myrcene, limonene, a-pinene, 1,4-cyclohexadiene, 1,5,5-trimethyl-6-methylene-cyclohexene, 3,7,11,15-tetramethyl-2-hexadecen-1-ol,n-hexadecanoic acid and α-caryophyllene. The precision of the method was studied by five replicate analysis of the main valatile compounds in celery and a relative standard deviation value less than 5.0% showed that the method could provide good reproducibility.