Analysis of the volatile constituents of Apium graveolens L. and Oenanthe L. by gas chromatography-mass spectrometry, using headspace solid-phase microextraction

摘要

Gas chromatography-mass spectrometry with headspace solid-phase microextraction was used for the analysis of the volatile constituents of celery and water dropwort. Solid-phase microextraction was carried out with a 65 pm polydimethylsiloxane-divinylbenzene fiber at 60degreesC for 45 min. The fiber was desorbed in the GC injection liner at 250degreesC for 3 min. Thirty-eight compounds in celery and thirty-five compounds in water dropwort were separated and identified. The main compounds found in both celery and water dropwort included cis-3-hexen-1-ol, alpha-myrcene, limonene, a-pinene, 1,4-cyclohexodiene, 1,5,5-trimethyl-6-methylene-cyclohexene, 3,711,15-tetramethyl-2-hexadecen-1-ol, n-hexadecanoic acid and alpha-caryophyllene. The precision of the method was studied by five replicate analysis of the main volatile compounds in celery and a relative standard deviation value less than 5.0% showed that the method could provide good reproducibility. [References: 15]