Influence of recombinant retroviral vector expressing antisense GTFα on malignant phenotype of human pancreatic carcinoma cell line

摘要

Conclusion the antisense TGFα expressing vector can block the target gene expression, suppress the cell growth and partially reverse the malignant phe- notype of pancreatic carcinoma cells. Objective to observe the effects of antisense TGFα on the growth of human pancreatic carcinoma cell line cells. Methods A recombinant retroviral vector ex- pressing antisense TGFαwas constructed, and trans- fected the ecotropic packaging cell line ψ-2 with lipo- fectin. After the amphotropic packaging cell line PA317 was transfected with the virus supernatant of ψ-2, the replication-defective, amphotropic retroviral supernatant was used to infect human pancreatic carcinoma cell line PC-7.