Investigation on mechanism of Cr(Ⅵ) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil

摘要

A strain CSB 9 isolated from chromite mine soil of Sukinda, India was identified as Bacillus amyloliquefac-iens based on biochemical and 16S rRNA gene sequencing. The strain exhibited relatively high tolerance to Cr(Ⅵ) (≤900 mg L~(-1)) and fast reduction rate of 2.22 mg Cr(Ⅵ) L~(-1) h~(-1), under optimized conditions of 100 mg L~(-1) Cr(Ⅵ), pH 7 and temperature 35 ℃ within 45 h. Mechanism of Cr(Ⅵ) reduction as well as nature and fate of the reduced product were studied to determine the scope of removal of reduced Cr(Ⅲ) end product. AAS analyses of the culture products treated with Cr(Ⅵ) for 45 h showed the distribution of Cr(Ⅲ) in pellet and culture supernatant to be 37.4 ±1.7 and 62.6 ± 3.4 mg L~(-1), respectively. In SEM images, the bacterial pellets with Cr(Ⅵ) treatment appeared coagulated, rough and porous whereas the pellets without Cr(Ⅵ) treatment appeared regular, smooth and non-porous in structure. SEM-EDX of the bacterial precipitates under Cr(Ⅵ) treatment revealed immobilization of Cr(Ⅲ) species on the bacterial cell surface. Further Raman spectroscopy analysis confirmed the presence of Cr(Ⅲ) species, with characteristic peak at around 600 cm~(-1). TEM-EDX study of the bacterial precipitates under Cr(Ⅵ) treatment showed intracellular deposition of Cr(Ⅲ) which are in nanometric range. Further characterization of reduced product by XRD, FT-IR and SAED analyses suggested the formation of poorly crystalline end products. A Cr(Ⅵ) removal mechanism considering both the surface immobilization and intracellular accumulation of Cr(Ⅲ) along with the formation of coagulated cell precipitate by living B. amyloliquefac-iens was suggested.