Analysis of Norditerpenoid Alkaloids Extracted from Aconitum sinomantanum Nakai by Electrospray Ionization Tandem Mass Spectrometry

摘要

Electrospray ionization mass spectrometry(ESI-MS) was applied simultaneously in determining norditerpenoid alkaloids from the roots of Aconitum sinomantanum Nakai (RAS) based on molecular mass information.The tandem mass spectra (ESI-MS") provided the alkaloidal structural information,through which the existence of these alkaloids was further confirmed.Accordingly,six known norditerpenoid alkaloids were simultaneously determined on the basis of their ESI-MS" spectra.Furthermore,based on the diagnostic fragmentation pathways of alkaloidal MS~n,a rapid method for direct detection and characterization of alkaloids from an ethanolic extract of RAS was described.