Analysis of Norditerpenoid Alkaloids Extracted from Aconitum sinomantanum Nakai by Electrospray Ionization Tandem Mass Spectrometry

摘要

Electrospray ionization mass spectrometry(ESI-MS) was applied simultaneously in determining norditerpenoid alkaloids from the roots of Aconitum sinomantanum Nakai(RAS) based on molecular mass information. The tandem mass spectra(ESI-MS n) provided the alkaloidal structural information, through which the existence of these alkaloids was further confirmed. Accordingly, six known norditerpenoid alkaloids were simultaneously determined on the basis of their ESI-MS n spectra. Furthermore, based on the diagnostic fragmentation pathways of alkaloidal MS n, a rapid method for direct detection and characterization of alkaloids from an ethanolic extract of RAS was described.