In vivo multimode Raman imaging reveals concerted molecular composition and distribution changes during yeast cell cyclef

摘要

In vivo time-lapse Raman imaging reveals highly dynamic and concerted changes in concentration and distribution of phospho-lipids and proteins during and after cell division of a single living Schizosaccharomyces pombe cell. The cell cycle is central to the reproduction of all living organisms. In cell division, the final phase of the cell cycle, vital cellular components including nucleic acids, proteins, membranes and organelles are duplicated accurately and inherited equally by the two daughter cells. This process is exceedingly dynamic in nature in the sense that molecular composition and distribution change continuously before, during and after the cell division. A variety of biochemical and bioanalytical approaches have been developed and employed to elucidate underlying molecular mechanisms of the cell cycle (particularly those of the cell-cycle control). However, these methods lack time and space specificity and as a result, the information yielded by those methods is inevitably time- and space-averaged.