Cloning and analysing of 5' flanking region of Xenopus organizer gene noggin

摘要

Xenopus organizer specific gene Noggin possesses nearly all the characteristics properties of the action of or- ganizer to specify the embryonic body axis. To analyze how the maternal inherited factors control its expression pattern, we cloned the 5' regulatory region of noggin gene. The 1.5 kb upstream sequense could direct reporter gene To express in vivo and data from deletion analysis indi- Cated that a 229 base pair fragment is essential for acti- Vating noggin expression.