Inhibitory effect of endostatin expressed by human liver carcinoma SMMC7721 on endothelial cell proliferation in vitro

摘要

Conclusion: Human endostatin can be stably expressed by SMMC7721 cell transferred with human endostain gene and its product can significantly inhibit the proliferation of human umbilical vein endothelial cell in vitro. Aim: To construct a stable transfectant of human liver Carcinoma cell line SMMC7721 that could secret human Endostatin and to explore the effect of human endostatin Expressed by the transfectant on endothelial cell proliferation. Methods: Recombinant retroviral plasmid pLnck-Endo Containing the cDNA for human endostain gene together With rat albumin signal peptide was engineered and Transferred into SMMC7721 cell by lipofectamine.